1E4B
L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant N29Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-05-15 |
| Detector | MULTIWIRE SIEMENS |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 93.650, 93.650, 43.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.840 |
| R-factor | 0.165 * |
| Rwork | 0.165 |
| R-free | 0.20400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.900 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.054 * | 0.170 * |
| Number of reflections | 16459 | 1428 * |
| <I/σ(I)> | 10.4 | 4.4 |
| Completeness [%] | 97.0 | 91 |
| Redundancy | 3.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 293 * | Dreyer, M.K., (1996) Acta Crystallog. sect., D52, 1082. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.5 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.62 (M) | |
| 6 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 7 | 1 | reservoir | 4 (mM) | ||
| 8 | 1 | reservoir | beta-mercaptoethanol | 4 (mM) |
