1E48
L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73Q/Y113F/Y209F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-11-15 |
| Detector | MULTIWIRE SIEMENS |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 93.700, 93.700, 43.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.970 |
| R-factor | 0.171 * |
| Rwork | 0.171 |
| R-free | 0.22100 |
| Structure solution method | OTHER |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.054 * | 0.190 * |
| Number of reflections | 13420 | 1143 * |
| <I/σ(I)> | 12.4 | 4 |
| Completeness [%] | 96.0 | 89 |
| Redundancy | 4.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 293 * | Dreyer, M.K., (1996) Acta Crystallog. sect., D52, 1082. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.5 (mM) | ||
| 4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.62 (M) | |
| 6 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 7 | 1 | reservoir | 4 (mM) | ||
| 8 | 1 | reservoir | beta-mercaptoethanol | 4 (mM) |
