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1E46

L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73S

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]293
Detector technologyAREA DETECTOR
Collection date1996-10-15
DetectorMULTIWIRE SIEMENS
Spacegroup nameP 4 21 2
Unit cell lengths94.100, 94.100, 43.500
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution10.000 - 2.550
R-factor0.149

*

Rwork0.149
R-free0.22900
Structure solution methodOTHER
RMSD bond length0.014
RMSD bond angle0.044
Data reduction softwareXDS
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]10.0002.630
High resolution limit [Å]2.5502.550
Rmerge0.064

*

0.023

*

Number of reflections6347521

*

<I/σ(I)>10.83.3
Completeness [%]95.089
Redundancy5.33.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.6

*

293

*

Dreyer, M.K., (1996) Acta Crystallog. sect., D52, 1082.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein10 (mg/ml)
21dropTris-HCl20 (mM)
31drop0.5 (mM)
41dropbeta-mercaptoethanol10 (mM)
51reservoirammonium sulfate1.62 (M)
61reservoirpotassium phosphate20 (mM)
71reservoir4 (mM)
81reservoirbeta-mercaptoethanol4 (mM)

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