1E3P
tungstate derivative of Streptomyces antibioticus PNPase/GPSI enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MSC |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-15 |
Detector | R-AXIS IV |
Spacegroup name | H 3 2 |
Unit cell lengths | 130.830, 130.830, 328.732 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.840 - 2.500 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e3h |
RMSD bond length | 0.006 |
RMSD bond angle | 23.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.080 * | |
Total number of observations | 462572 * | |
Number of reflections | 37642 | |
<I/σ(I)> | 17.7 | 2.8 |
Completeness [%] | 99.3 | 92.3 |
Redundancy | 6.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8.5 * | 20 * | 2.0M (NH4)2SO4, 100MM TRISHCL PH8.5, 100MM BISTRISHCL PH6.5, 60MM NACL, 4MM MGCL2, 5MM DTT, 50MM NA2W04, pH 7.00 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | 15 (mM) | ||
3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
6 | 1 | reservoir | 0.75 (mM) |