1E3O
Crystal structure of Oct-1 POU dimer bound to MORE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-11-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.300, 52.400, 69.000 |
Unit cell angles | 90.00, 127.60, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.22 |
Rwork | 0.220 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oct |
RMSD bond length | 0.013 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.039 | 0.111 |
Number of reflections | 91810 | |
<I/σ(I)> | 24.4 | 6.2 |
Completeness [%] | 95.3 | 97 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 20 * | Remenyi, A., (2001) Acta Crystallogr., D57, 1634. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 22-24 (%(v/w)) | |
2 | 1 | reservoir | HEPES | 50 (mM) | |
3 | 1 | reservoir | spermine | 1.8 (mM) |