1E3H
SeMet derivative of Streptomyces antibioticus PNPase/GPSI enzyme
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9740, 0.9791, 0.98 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 133.610, 133.610, 344.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.990 - 2.600 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22600 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.990 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 36704 | |
| <I/σ(I)> | 5.3 | 1.1 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 20 * | 1.8M (NH4)2SO4, 100MM TRISHCL PH8.5, 100MM BISTRISHCL PH6.5, 60MM NACL, 4MM MGCL2, 5MM DTT., pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | 15 (mM) | ||
| 3 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 6 | 1 | reservoir | 0.75 (mM) |
