1E31

SURVIVIN DIMER H. SAPIENS

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-15
Detector	MARRESEARCH
Wavelength(s)	1.27557, 1.28243, 1.28283, 1.60478
Spacegroup name	I 1 2 1
Unit cell lengths	82.220, 71.050, 89.450
Unit cell angles	90.00, 96.12, 90.00

Refinement procedure

Resolution	27.760 - 2.710
R-factor	0.235
Rwork	0.235
R-free	0.27000
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.064	0.229
Number of reflections	12978
<I/σ(I)>	9.66	2.51
Completeness [%]	92.8	75.9
Redundancy	2.46 *	1.77 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *		3 % PEG MME 2K, 2 MM HEXAMINE COBALT, 100 MM HEPES PH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES
2	1	reservoir	PEG2000 MME	4 (%)
3	1	reservoir	ethylene glycol	5 (%)
4	1	reservoir	hexamine cobalt chloride	1 (mM)