1E30
Crystal structure of the Met148Gln mutant of rusticyanin at 1.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-15 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.861, 61.380, 53.330 |
| Unit cell angles | 90.00, 96.19, 90.00 |
Refinement procedure
| Resolution | 52.700 - 1.500 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.203 |
| Total number of observations | 62663 * | |
| Number of reflections | 33706 | |
| <I/σ(I)> | 12 | 3.1 |
| Completeness [%] | 76.5 | 70.7 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | 293 * | drop was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 30 (%) | |
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | citric acid | 50 (mM) |
