1E2U
Low Temperature Structure of Hybrid Cluster Protein from Desulfovibrio vulgaris to 1.6A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX7.2 |
Synchrotron site | SRS |
Beamline | PX7.2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.630, 64.680, 152.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.600 |
Rwork | 0.160 |
R-free | 0.18300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e1d |
RMSD bond length | 0.007 |
RMSD bond angle | 0.021 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.680 |
High resolution limit [Å] | 1.590 | 1.590 |
Rmerge | 0.070 * | 0.100 * |
Number of reflections | 77446 | |
<I/σ(I)> | 5.6 | 6.5 |
Completeness [%] | 91.3 | 87.7 |
Redundancy | 2.5 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.9 | 277 * | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | |
3 | 1 | reservoir | MES | 0.1 (M) | precipitant |
4 | 1 | reservoir | magnesium acetate | 0.2 (M) | precipitant |
5 | 1 | reservoir | PEG8000 | 25-30 (%) | precipitant |