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1E2U

Low Temperature Structure of Hybrid Cluster Protein from Desulfovibrio vulgaris to 1.6A

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSRS BEAMLINE PX7.2
Synchrotron siteSRS
BeamlinePX7.2
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1998-06-15
DetectorMARRESEARCH
Spacegroup nameP 21 21 21
Unit cell lengths63.630, 64.680, 152.290
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 1.600
Rwork0.160
R-free0.18300

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1e1d
RMSD bond length0.007
RMSD bond angle0.021
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.680
High resolution limit [Å]1.5901.590
Rmerge0.070

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0.100

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Number of reflections77446
<I/σ(I)>5.66.5
Completeness [%]91.387.7
Redundancy2.52.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

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5.9277

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drop consists of equal amounts of protein and precipitant solutions

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Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein12 (mg/ml)
21dropTris-HCl5 (mM)
31reservoirMES0.1 (M)precipitant
41reservoirmagnesium acetate0.2 (M)precipitant
51reservoirPEG800025-30 (%)precipitant

217705

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