1E2O

CATALYTIC DOMAIN FROM DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (3 Å)

Cell axes222.800222.800222.800
Cell angles90.0090.0090.00
SpacegroupF 4 3 2
Resolution limits20.00 - 3.00
the highest resolution shell value3.190 - 3.000
R-factor0.205
R-work0.20500
the highest resolution shell value0.261
R-free0.24900
the highest resolution shell value0.341
RMSD bond length0.010
RMSD bond angle1.500 (24.700*)

Data Collection Statistics

Resolution limits20.00 - 3.00
the highest resolution shell value -
Number of reflections9906
Rmerge_l_obs0.046*
the highest resolution shell value0.145*
Completeness98.5
the highest resolution shell value100.*
Redundancy4.1
the highest resolution shell value4.5
I/sigma(I)3

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1Vapor diffusion, hanging drop*7 (7.3*)K*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein20 (mg/ml)
21droppotassium phosphate50 (mM)
31dropdithiothreitol1 (mM)
41dropEDTA1 (mM)
51dropbenzamidine1 (mM)
61reservoirammonium sulfate1.2 (M)
71reservoirethanol1 (%(v/v))
Annotated Information is extracted from Literature Info*