1E20
The FMN binding protein AtHal3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF NONIUS |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 63 |
Unit cell lengths | 112.309, 112.309, 33.182 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.000 - 2.020 |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.26300 * |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 0.034 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.600 * | 2.120 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.091 * | 0.404 * |
Number of reflections | 15729 | |
<I/σ(I)> | 6 | 1.8 |
Completeness [%] | 97.1 | 90.5 * |
Redundancy | 2.9 * | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 * | 2.5 M AMMONIUM SULPHATE, PH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.1 (M) | |
3 | 1 | drop | imidazole | 0.1 (M) | |
4 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
6 | 1 | reservoir | ammonium sulfate | 2.5 (M) |