1E1V

HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE INHIBITOR NU2058

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	52.620, 71.050, 71.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.950
R-factor	0.195 *
Rwork	0.195
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hck
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.040
High resolution limit [Å]	1.950	1.950
Rmerge	0.065	0.313
Total number of observations	42243 *
Number of reflections	17940
<I/σ(I)>	12.3	1.6
Completeness [%]	88.8	84.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	7.4		Lawrie, A.M., (1997) Nature Struct. Biol., 4, 796. *