1E1M
ADRENODOXIN REDUCTASE in complex with NADPH obtained by a soaking experiment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Collection date | 1999-04-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.500, 62.500, 78.600 |
| Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.850 |
| Rwork | 0.188 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.400 * |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.044 * | 0.150 * |
| Number of reflections | 47193 * | |
| <I/σ(I)> | 12.3 | 5 |
| Completeness [%] | 96.0 * | 91 * |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | glycerol | 5 (%) | |
| 3 | 1 | drop | PEG8000 | 8 (%(w/v)) | |
| 4 | 1 | drop | sodium cacodylate | 50 (mM) | Buffer C |
| 5 | 1 | drop | calcium acetate | 100 (mM) | BufferC |
| 6 | 1 | reservoir | Buffer C | ||
| 7 | 1 | reservoir | PEG8000 | 12 (%(w/v)) |
