1E1L
STRUCTURE OF ADRENODOXIN REDUCTASE IN COMPLEX WITH NADP obtained by cocrystallisation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.500, 62.800, 85.300 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| Rwork | 0.219 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cjc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.900 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.070 | 0.364 |
| Number of reflections | 28171 | |
| <I/σ(I)> | 8.7 | 3.4 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | DROPLET: 4 MG/ML PROTEIN, 8% PEG8000, 5% GLYCEROL, 100 MM CALCIUM ACETATE, 50 MM SODIUM-CACODYLATE PH 6.5. RESERVOIR: 12 % PEG8000, 100 MM CALCIUM ACETATE, 50 MM SODIUM CACODYLATE PH 6.5, 0.05 MM NADP+. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | glycerol | 5 (%) | |
| 3 | 1 | drop | PEG8000 | 8 (%(w/v)) | |
| 4 | 1 | drop | sodium cacodylate | 50 (mM) | Buffer C |
| 5 | 1 | drop | calcium acetate | 100 (mM) | BufferC |
| 6 | 1 | reservoir | Buffer C | ||
| 7 | 1 | reservoir | PEG8000 | 12 (%(w/v)) |
