1E1E

Crystal structure of a Monocot (Maize ZMGlu1) beta-glucosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Collection date	1999-01-15
Spacegroup name	P 1 21 1
Unit cell lengths	62.880, 118.340, 77.100
Unit cell angles	90.00, 90.30, 90.00

Refinement procedure

Resolution	27.000 - 2.500
R-factor	0.24 *
Rwork	0.256
R-free	0.28300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cbg
RMSD bond length	0.009
RMSD bond angle	23.100 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000 *	2.420
High resolution limit [Å]	2.420	2.340
Rmerge	0.093 *	0.276 *
Total number of observations	153615 *
Number of reflections	42762 *
<I/σ(I)>	6.4	2.3
Completeness [%]	99.9 *	99.9 *
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		0.2 M AMMONIUM SULFATE, pH 5.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	11 (mg/ml)
2	1	drop	HEPES	20 (mM)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	ammonium acetate	0.2 (M)
5	1	reservoir	PEG4000	27 (%)