1E1D
Hybrid Cluster Protein from Desulfovibrio vulgaris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.500, 65.500, 155.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.720 |
| Rwork | 0.171 |
| R-free | 0.20800 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.011 |
| Data reduction software | MOSFLM |
| Data scaling software | Agrovata |
| Phasing software | MLPHARE |
| Refinement software | RESTRAIN |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.880 | 1.770 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.036 * | 0.162 * |
| Number of reflections | 70418 | |
| <I/σ(I)> | 10.5 | 3.7 |
| Completeness [%] | 97.2 | 81.3 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.9 | 277 * | Arendsen, A., (1996) Acta Crystallogr. D.52, 1211. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | MES | 0.1 (M) | |
| 5 | 1 | drop | magnesium acetate | 66 (mM) | |
| 6 | 1 | reservoir | MES | 0.1 (M) | |
| 7 | 1 | reservoir | magnesium acetate | 66 (mM) |
