1E0Y
Structure of the D170S/T457E double mutant of vanillyl-alcohol oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-15 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 131.281, 131.281, 134.355 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.750 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vao |
RMSD bond length | 0.009 |
RMSD bond angle | 0.036 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | CCP4 |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.110 | 0.371 * |
Total number of observations | 69063 * | |
Number of reflections | 28714 | |
<I/σ(I)> | 6.4 | 2.7 |
Completeness [%] | 97.3 | 95 |
Redundancy | 2.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | pH 4.60 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | reservoir | sodium acetate/hydrochloride | 100 (mM) | |
4 | 1 | reservoir | PEG4000 | 3 (%(w/v)) |