1E0X
XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.210, 81.060, 72.810 |
Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.650 |
R-factor | 0.12 * |
Rwork | 0.126 |
R-free | 0.16200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NATIVE STRUCTURE AT 1.2 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.028 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.033 | 0.097 |
Number of reflections | 66881 | |
<I/σ(I)> | 35.6 | 12.9 |
Completeness [%] | 99.0 | 94 |
Redundancy | 3.5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | PROTEIN WAS CRYSTALLISED WITH 18 % PEG 5000 AS PRECIPITANT, 100MM HEPES PH 7.5 AS BUFFER, 10% ISOPROPANOL, CRYSTAL WERE SOAKED IN PRESENCE OF POWDERED SUBSTRATE FOR 12 HOURS. 15% GLYCEROL WAS ADDED AS CRYOPROTECTANT |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium HEPES | 0.1 (M) | |
2 | 1 | reservoir | PEG5000 | 18 (%(w/v)) | |
3 | 1 | drop | protein | 30 (mg/ml) | |
4 | 1 | drop | isopropanol | 10 (%(v/v)) |