1E0S

small G protein Arf6-GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	55.660, 55.660, 194.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.280
R-factor	0.172
Rwork	0.172
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hur
RMSD bond length	0.015
RMSD bond angle	2.211
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (98)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.280	2.280
Rmerge	0.064 *	0.152 *
Total number of observations	76362 *
Number of reflections	8748
<I/σ(I)>	12.8
Completeness [%]	98.5	98.5
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5	4 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.8 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	Tris-HCl	0.1 (M)