1E0R
Beta-apical domain of thermosome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.320, 57.320, 106.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.261 |
| Rwork | 0.261 |
| R-free | 0.31800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ASS WITHOUT I245-K274 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.041 | 0.393 |
| Total number of observations | 19163 * | |
| Number of reflections | 5170 | |
| <I/σ(I)> | 27 | 3.3 |
| Completeness [%] | 97.8 | 96.8 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 9.1 | 20 * | pH 9.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG20000 | 8 (%(w/v)) | |
| 2 | 1 | reservoir | dioxane | 2 (%(v/v)) | |
| 3 | 1 | reservoir | bicine | 0.1 (M) | pH9.1 |
| 4 | 1 | drop | protein | 10 (mg/ml) |
