1E0P

L intermediate of bacteriorhodopsin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	110
Detector technology	CCD
Collection date	1999-10-15
Detector	MARRESEARCH
Spacegroup name	P 63
Unit cell lengths	60.960, 60.960, 109.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.500 - 2.100
R-factor	0.265
Rwork	0.265
R-free	0.28800
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	18.700 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.500	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.063 *	0.521 *
Total number of observations	73411 *
Number of reflections	13088
<I/σ(I)>	9	1.4
Completeness [%]	96.6	99.2
Redundancy	5.6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6	20 *	Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium potassium Pi	3.3 (M)
2	1	1	protein	3.5 (mg/ml)
3	1	1	methylpentandiol	0.05 (%)
4	1	1	beta-octylglycopyranoside	1.2 (%)