1E0K
gp4d helicase from phage T7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 120.650, 120.650, 284.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.300 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.30700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 * |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.057 * | 0.332 * |
| Number of reflections | 30597 | |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 94.5 * | 96.9 |
| Redundancy | 2.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 100 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | CHES-NaOH | 50 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 0.8 (M) | |
| 7 | 1 | reservoir | sodium azide | 1 (mM) | |
| 8 | 1 | reservoir | PEG4000 | 20-30 (%(w/v)) | |
| 9 | 1 | reservoir | glycerol | 5 (%(v/v)) |
