1E0C
SULFURTRANSFERASE FROM AZOTOBACTER VINELANDII
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 40.310, 150.310, 53.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23000 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 * | 0.230 |
| Total number of observations | 328605 * | |
| Number of reflections | 30532 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 98.5 | 93.6 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 1.8 M MGSO4 50 MM MES PH 6.0, 5% (V/V)ETHANEDIOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 30 (mM) | |
| 3 | 1 | reservoir | 3 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 5 (mM) |
