1E0B

Chromo shadow domain from fission yeast swi6 protein.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Collection date	1999-05-15
Spacegroup name	P 32 2 1
Unit cell lengths	59.890, 59.890, 91.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.000 - 1.900
R-factor	0.226
Rwork	0.226
R-free	0.25900
Structure solution method	MIR
RMSD bond length	0.029
RMSD bond angle	2.282
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall
Low resolution limit [Å]	22.250
High resolution limit [Å]	1.900
Rmerge	0.061
Total number of observations	115148 *
Number of reflections	115148
Completeness [%]	99.8
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	4 *	20% (W/V) PEG 4000, 0.2M SODIUM ACETATE, 0.1M TRIS HCL PH 8.5, TEMPERATURE 4 DEGREES CENTIGRADE PROTEIN CONCENTRATION 10 MG/ML TIME 3 TO 4 DAYS

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.2 (M)
4	1	reservoir	Tris-HCl	0.1 (M)