1E06
Porcine Odorant Binding Protein Complexed with 5-methyl-2-(1-methylethyl)phenol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.076, 88.287, 93.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.120 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.25700 * |
Structure solution method | OTHER |
RMSD bond length | 0.008 |
RMSD bond angle | 27.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.000 | 2.150 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.029 * | 0.168 * |
Number of reflections | 23430 | |
<I/σ(I)> | 15 | 5.1 |
Completeness [%] | 99.0 | 99.3 |
Redundancy | 3.2 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 | 20 * | 2M AMMONIUM SULFATE, AND 5% ISOPROPANOL, pH 7.80 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
3 | 1 | reservoir | iso-propanol | 5 (%(v/v)) |