1E05

PLASMA ALPHA ANTITHROMBIN-III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	61.410, 98.310, 90.410
Unit cell angles	90.00, 103.32, 90.00

Refinement procedure

Resolution	20.000 - 2.620
R-factor	0.207 *
Rwork	0.196
R-free	0.25800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ant
RMSD bond length	0.007
RMSD bond angle	0.023
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.620
Rmerge	0.048
Number of reflections	23397
Completeness [%]	72.0
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.4 *		CRYSTALS GROWN IN MICROGRAVITY NASA SPACE SHUTTLE MISSION STS-67 20MG/ML LATENT ANTITHROMBIN-III IN 20MM TRIS HCL PH 8.0 CRYSTALLIZED IN 19% PEG 4000, 50 MM NA/K PHOSPHATE PH 6.7, 0.05% AZIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris-HCl	20 (mM)
3	1	2	PEG4000	16 (%(w/v))
4	1	2	sodium potassium phosphate	50 (mM)