1DZO
Truncated PAK pilin from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 38.114, 38.114, 149.775 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.440 - 1.630 |
| Rwork | 0.153 |
| R-free | 0.18100 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.023 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | ARP/wARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.440 | 1.720 |
| High resolution limit [Å] | 1.633 | 1.630 |
| Rmerge | 0.049 * | 0.190 * |
| Total number of observations | 110628 * | |
| Number of reflections | 14500 * | |
| <I/σ(I)> | 26.7 | 10.1 |
| Completeness [%] | 99.5 | 96.4 |
| Redundancy | 7.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.2 | HANGING DROP USING 1 ML OF RESERVOIR DROPS MADE FROM 3 MICROLITRE PROTEIN AND 3 MICROLITRE OF MOTHER LIQUOR PROTEIN SOLUTION = 10 MG/ML IN WATER MOTHER LIQUOR = 60% (NH4)2SO4, 0.1M HEPES PH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 60 (%) | |
| 3 | 1 | 1 | HEPES | 0.1 (M) | |
| 4 | 1 | 2 | ammonium sulfate | 60 (%) | |
| 5 | 1 | 2 | HEPES | 0.1 (M) |
