1DZN
Asp170Ser mutant of vanillyl-alcohol oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 |
| Unit cell lengths | 131.330, 131.330, 134.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.22686 |
| Rwork | 0.227 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.043 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.128 | 0.323 * |
| Total number of observations | 23315 * | |
| Number of reflections | 16730 | |
| <I/σ(I)> | 4.7 | 4.6 |
| Completeness [%] | 83.7 | 83.8 * |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 4.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | sodium acetate/hydrochloride | 50 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 5 (%(w/v)) |
