1DZM
Porcine Odorant Binding Protein Complexed with benzoic acid phenylmethylester
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-24 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.009, 88.679, 93.163 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.930 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.25600 * |
Structure solution method | OTHER |
RMSD bond length | 0.015 |
RMSD bond angle | 1.660 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.053 * | 0.258 * |
Number of reflections | 26679 | |
<I/σ(I)> | 13.7 | 3.3 |
Completeness [%] | 99.2 | 99.9 |
Redundancy | 3.35 * | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 | 20 * | PROTEIN (0.47MM) IS INCUBATED WITH 10MM OF PYRAZINE, THE COMPLEX WAS CRYSTALLISED FROM 2M AMMONIUM SULFATE, AND 5% ISOPROPANOL IN HANGING DROPS, AT 20 DEGREES, pH 7.80 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
3 | 1 | reservoir | iso-propanol | 5 (%(v/v)) |