1DZK
Porcine Odorant Binding Protein Complexed with pyrazine (2-isobutyl-3-metoxypyrazine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-05 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.169, 88.385, 93.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 1.480 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.24200 |
Structure solution method | OTHER |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.510 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.056 * | 0.093 * |
Number of reflections | 56300 | |
<I/σ(I)> | 14 | 1.8 |
Completeness [%] | 92.6 | 92.3 |
Redundancy | 2.72 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.8 | 20 * | PROTEIN (0.47MM) IS INCUBATED WITH 10MM OF PYRAZINE., pH 7.80 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 2 (M) | |
3 | 1 | reservoir | iso-propanol | 5 (%(v/v)) |