1DZK

Porcine Odorant Binding Protein Complexed with pyrazine (2-isobutyl-3-metoxypyrazine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-03-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.169, 88.385, 93.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.480
R-factor	0.226
Rwork	0.226
R-free	0.24200
Structure solution method	OTHER
RMSD bond length	0.012
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.510
High resolution limit [Å]	1.480	1.480
Rmerge	0.056 *	0.093 *
Number of reflections	56300
<I/σ(I)>	14	1.8
Completeness [%]	92.6	92.3
Redundancy	2.72	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	20 *	PROTEIN (0.47MM) IS INCUBATED WITH 10MM OF PYRAZINE., pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	iso-propanol	5 (%(v/v))