1DZ8
oxygen complex of p450cam from pseudomonas putida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 88 |
Detector technology | CCD |
Collection date | 1997-10-15 |
Detector | BRANDEIS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.000, 61.800, 94.700 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.800* |
R-factor | 0.203 * |
Rwork | 0.174 |
R-free | 0.24800 |
Structure solution method | OTHER |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.790 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.092 * | 0.388 * |
Total number of observations | 217362 * | |
Number of reflections | 69362 | |
<I/σ(I)> | 9.5 | 2.2 |
Completeness [%] | 96.6 | 85 * |
Redundancy | 3.1 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 275 | CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 30 (mg/ml) | |
2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.4 |
3 | 1 | reservoir | 250 (mM) | ||
4 | 1 | reservoir | DTE | 100 (mM) | |
5 | 1 | reservoir | camphor | 1 (mM) | |
6 | 1 | reservoir | PEG4000 | 27-30 (%) |