1DZ6
ferrous p450cam from pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.800, 62.800, 95.500 |
| Unit cell angles | 90.00, 90.60, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.900 |
| R-factor | 0.211 * |
| Rwork | 0.188 |
| R-free | 0.25200 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 0.042 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.600 * |
| Rmerge | 0.087 * | 0.343 * |
| Total number of observations | 234827 * | |
| Number of reflections | 61973 | |
| <I/σ(I)> | 11.6 | 2.7 |
| Completeness [%] | 97.6 | 91.8 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 275 | CRYSTALS WERE GROWN USING THE SITTING DROP GEOMETRY AT 2 DEG. C. 5 UL OF 30 MG/ML P450 IN 50 MM TRIS HCL, 250 MM KCL, 0.5 MM CAMPHOR WERE MIXED WITH AN EQUAL VOLUME OF THE RESERVOIR SOLUTION (27-30% PEG 4000, 100 MM DTE, SAME BUFFER AS PROTEIN)., pH 7.40 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | DTE | 100 (mM) | |
| 5 | 1 | reservoir | camphor | 1 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 27-30 (%) |
