1DZ4
ferric p450cam from pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.400, 62.700, 95.500 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| Rwork | 0.186 |
| R-free | 0.23700 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.064 | 0.227 * |
| Total number of observations | 242005 * | |
| Number of reflections | 101046 | |
| <I/σ(I)> | 9.4 | 2.4 |
| Completeness [%] | 96.0 | 95.4 |
| Redundancy | 2.39 | 2.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 275 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | DTE | 100 (mM) | |
| 5 | 1 | reservoir | camphor | 1 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 27-30 (%) |
