1DZ3
DOMAIN-SWAPPING IN THE SPORULATION RESPONSE REGULATOR SPO0A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1998-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.024, 59.024, 66.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.000 - 1.650 |
| R-factor | 0.23538 |
| Rwork | 0.208 |
| R-free | 0.26593 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.056 * | 0.268 * |
| Total number of observations | 143244 * | |
| Number of reflections | 16570 | |
| <I/σ(I)> | 24.8 | 3.8 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 8.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 18 * | 10 % PEG 4000, 100 MM LITHIUM SULPHATE 100 MM SODIUM ACETATE, PH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 5 | 1 | reservoir | lithium sulfate | 100 (mM) |
