1DYO
Xylan-Binding Domain from CBM 22, formally x6b domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.490, 89.490, 207.690 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.036 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.074 | 0.437 |
| Number of reflections | 141380 | |
| <I/σ(I)> | 19.2 | 3.4 |
| Completeness [%] | 98.0 | 96.5 |
| Redundancy | 5.11 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | CRYSTALS WERE GROWN FROM A PROTEIN CONCENTRATION OF 50 MG/ML IN 0.1 M NAAC BUFFER, PH 4.6, CONTAINING 10 MM DTT, 25 % (V/V) GLYCEROL AND WITH 12 % (W/V) PEG 8000 AS THE PRECIPITANT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | 0.1 (mg/ml) | ||
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | glycerol | 25 (%(v/v)) | |
| 5 | 1 | drop | PEG8000 | 12 (%(w/v)) |
