1DY5
Deamidated derivative of bovine pancreatic ribonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.314, 37.470, 44.915 |
| Unit cell angles | 90.00, 94.55, 90.00 |
Refinement procedure
| Resolution | 61.000 - 0.870 |
| R-factor | 0.1 * |
| R-free | 0.11980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lsq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.038 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.000 | 0.880 |
| High resolution limit [Å] | 0.870 | 0.870 |
| Rmerge | 0.053 | 0.104 |
| Total number of observations | 493374 * | |
| Number of reflections | 160386 | |
| <I/σ(I)> | 25.5 | 6.4 |
| Completeness [%] | 96.6 | 68.8 |
| Redundancy | 3.1 | 1.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.7 | 20 * | Capasso, S., (1996) J.Mol.Biol., 257, 492. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | acetic acid | 0.1 (M) | |
| 2 | 1 | 1 | protein | 20 (mg/ml) | lyophilised |
| 3 | 1 | 1 | ammonia |
