1DXS

Crystal structure of the C-terminal sterile alpha motif (SAM) domain of human p73 alpha splice variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-15
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	32.020, 32.020, 133.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.000 - 2.540
R-factor	0.275
Rwork	0.275
R-free	0.34600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cok
RMSD bond length	0.007
RMSD bond angle	20.400 *
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	2.710
High resolution limit [Å]	2.540	2.540
Rmerge	0.073	0.408
Number of reflections	2510
<I/σ(I)>	7.6	2
Completeness [%]	94.9	97.8
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	Wang, W.K., (2000) Acta Crystallogr.,Sect.D, 56, 769. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	ammonium dihydrogen orthophosphate	2.0 (M)