1DXI

STRUCTURE DETERMINATION OF GLUCOSE ISOMERASE FROM STREPTOMYCES MURINUS AT 2.6 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	137.650, 137.650, 132.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.214
RMSD bond length	0.020
RMSD bond angle	0.046
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.095 *
Number of reflections	31564 *
Completeness [%]	85.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GI solution	20 (mg/ml)
2	1	drop	ammonium sulphate	0.4 (M)
3	1	drop	magnesium sulfate	50 (mM)
4	1	drop	Tris-HCl
5	1	reservoir	ammonium sulfate	2.0 (M)
6	1	reservoir	Tris-HCl