1DXH
Catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Detector | BRUKER NONIUS |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 134.320, 134.320, 134.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ort |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.500 |
| Data reduction software | MADNESS |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.075 * | 0.258 * |
| Total number of observations | 80597 * | |
| Number of reflections | 13231 | |
| Completeness [%] | 95.4 | 65.5 * |
| Redundancy | 5.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.2 | pH 7.20 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.85 (M) | |
| 3 | 1 | reservoir | HEPES | 50 (mM) | pH7.2 |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | reservoir | PEG400 | 2 (%) | |
| 7 | 1 | reservoir | spermidine | 10 (mM) |
