1DWT
Photorelaxed horse heart MYOGLOBIN CO complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 88 |
| Detector technology | CCD |
| Collection date | 1997-10-09 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.800, 28.800, 35.200 |
| Unit cell angles | 90.00, 106.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1azi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.085 | 0.266 |
| Number of reflections | 23555 | |
| <I/σ(I)> | 7.4 | 2.6 |
| Completeness [%] | 97.8 | 98 |
| Redundancy | 2.4 | 2.42 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HHMB(SIGMA) WAS CRYSTALLIZED AT ROOM TEMPERATURE BY EQUILIBRATING 10 UL DROPS OF 5 MG/ML PROTEIN IN 1.7-1.8 M AMMONIUM SULFATE, 0.1 M TRIS HCL PH 7.5 USING THE HANGING DROP GEOMETRY |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.7-1.8 (M) | |
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.4-3.6 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
