1DVK
CRYSTAL STRUCTURE OF THE FUNCTIONAL DOMAIN OF THE SPLICING FACTOR PRP18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-10 |
| Detector | FUJI |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 49.935, 79.167, 171.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.150 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25200 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.985 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.041 | 0.097 |
| Total number of observations | 188477 * | |
| Number of reflections | 17664 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 93.2 | 55.7 |
| Redundancy | 10.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 17 * | 100mM MES 6.0, 25% PEG8000, 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | PEG8000 | 20 (%) |
