1DVB
RUBRERYTHRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.630, 81.550, 100.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.25500 |
| Structure solution method | COORDINATES FROM PDB |
| Starting model (for MR) | PDB ID 1RYT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.040 |
| Phasing software | SHELXS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.880 |
| Rmerge | 0.093 | |
| Total number of observations | 226047 * | |
| Number of reflections | 15420 | |
| Completeness [%] | 87.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 277 | deMare, F., (1996) Nat. Struct. Biol., 3, 539. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | TRIZMA hydrochloride | 0.1 (M) | |
| 2 | 1 | reservoir | PEG1450 | 15 (%) |
