1DV3
PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES IN THE CHARGE-SEPARATED D+QAQB-STATE WITH THE PROTON TRANSFER INHIBITOR CD2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.250, 141.250, 275.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.800 - 2.500 |
| R-factor | 0.227 |
| Rwork | 0.226 |
| R-free | 0.25200 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.650 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.800 | 2.560 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.086 | 0.330 |
| Total number of observations | 91189 * | |
| Number of reflections | 12363 * | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 94.5 | 78.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 25 * | PEG 4000, heptanetriol, TRIS-HCL, LDAO detergent, sodium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 10 (mM) | |
| 3 | 1 | drop | PEG4000 | 6.0 (%) | |
| 4 | 1 | drop | ampiphiles benzamidine hydrochloride | 0.4 (%) | |
| 5 | 1 | drop | heptane triol | 2.5 (%) | |
| 6 | 1 | reservoir | PEG4000 | 32 (%) |
