1DV1

STRUCTURE OF BIOTIN CARBOXYLASE (APO)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	1999-10-01
Detector	SIEMENS HI-STAR
Spacegroup name	P 21 21 21
Unit cell lengths	61.700, 95.800, 180.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.182
Rwork	0.180
R-free	0.23800
RMSD bond length	0.014
RMSD bond angle	17.500 *
Data scaling software	SAINT
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.990
High resolution limit [Å]	1.900	1.900
Rmerge	0.051	0.219
Total number of observations	235595 *
Number of reflections	78489	7871 *
<I/σ(I)>	15.7
Completeness [%]	92.1	71.9
Redundancy	3	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7	4 *	10mM potassium phosphate 1mM EDTA 2mM DTT, pH 7.0, MICRODIALYSIS, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	potassium phosphate	10 (mM)
3	1	2	EDTA	1 (mM)
4	1	2	dithiothreitol	2 (mM)
5	1	2		1 (mM)