1DUY
CRYSTAL STRUCTURE OF HLA-A*0201/OCTAMERIC TAX PEPTIDE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.340, 62.860, 74.840 |
| Unit cell angles | 82.01, 76.10, 77.96 |
Refinement procedure
| Resolution | 50.000 - 2.150 |
| R-factor | 0.1918 * |
| Rwork | 0.192 |
| R-free | 0.25400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.047 | 0.300 |
| Total number of observations | 60478 * | |
| Number of reflections | 38048 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 78.0 | 38.3 |
| Redundancy | 3.2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.4 * | 18 * | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tax8-A2 | 3 (mg/ml) | |
| 2 | 1 | drop | Tax8 peptide | 0.400 (mM) | |
| 3 | 1 | drop | MES | 25 (mM) | |
| 4 | 1 | drop | 0.1 (%) | ||
| 5 | 1 | reservoir | PEG6000 | 13 (%) | |
| 6 | 1 | reservoir | MES | 25 (mM) | |
| 7 | 1 | reservoir | 0.1 (%) |
