1DUX

ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-02-15
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	33.245, 140.523, 38.620
Unit cell angles	90.00, 115.52, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.202 *
Rwork	0.202
R-free	0.23600
RMSD bond length	0.005
RMSD bond angle	1.089
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	0.092
Number of reflections	17831
<I/σ(I)>	27.1
Completeness [%]	95.8	77.6
Redundancy	3.9	2.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	20 *	50 mM sodium Cacodylate, 10% PEG 2000, 100 mM MgCl2, 100 mM NaCl, 3 mM ZnCl2, pH 5.6, VAPOR DIFFUSION, temperature 293K
1	Vapor diffusion, hanging drop *	5.6	20 *	50 mM sodium Cacodylate, 10% PEG 2000, 100 mM MgCl2, 100 mM NaCl, 3 mM ZnCl2, pH 5.6, VAPOR DIFFUSION, temperature 293K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	sodium Cacodylate
2	1	1	MgCl2
3	1	1	NaCl
4	1	1	ZnCl2
5	1	1	PEG 2000
6	1	2	PEG 2000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.1 (mM)
10	1	reservoir		100 (mM)
11	1	reservoir		100 (mM)
12	1	reservoir		3.0 (mM)
2	1	drop	DNA	0.13 (mM)
3	1	drop	sodium cacodylate	25 (mM)
4	1	drop	PEG2000	5 (%(w/v))
5	1	drop		50 (mM)
6	1	drop		50 (mM)
7	1	drop		1.5 (mM)
8	1	reservoir	sodium cacodylate	50 (mM)
9	1	reservoir	PEG2000	10 (%(w/v))