1DUW

STRUCTURE OF NONAHEME CYTOCHROME C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-01
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	55.400, 55.400, 236.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.890
R-factor	0.183
Rwork	0.183
R-free	0.20400 *
RMSD bond length	0.010
RMSD bond angle	2.380
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.980
High resolution limit [Å]	1.890 *	1.890
Rmerge	0.079	0.187
Total number of observations	101894 *
Number of reflections	27269
<I/σ(I)>	9.7
Completeness [%]	87.9	80.6
Redundancy	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	drop consists of equal amounts of protein and precipitant solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	PEG6000	15 (%(w/v))	precipitant
3	1	reservoir	HEPES-NaOH	100 (mM)	precipitant