1DUS
MJ0882-A hypothetical protein from M. jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-31 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 69.340, 36.780, 68.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.570 - 1.800 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.23500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.077 | 0.320 |
| Number of reflections | 23819 | |
| <I/σ(I)> | 9.4 | |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.13 | 294 | 2.75 M ammonium sulfate, 0.1 M MES, pH 5.13, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | MES | 50 (mmol/L) | |
| 3 | 1 | drop | ammonium sulfate | 1.375 (mol/L) | |
| 4 | 1 | reservoir | MES | 0.1 (mol/L) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.75 (mol/L) |
