1DUQ
CRYSTAL STRUCTURE OF THE REV BINDING ELEMENT OF HIV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-26 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.993, 53.930, 64.729 |
| Unit cell angles | 114.52, 89.88, 102.85 |
Refinement procedure
| Resolution | 19.770 - 2.100 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.26900 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.380 | 0.248 |
| Number of reflections | 15998 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 94.7 | 83 * |
| Redundancy | 1.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.1M SODIUM CACODYLATE, PH 6.5, 0.2 M NACL, 35% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EDTA | 0.1 (mM) | |
| 2 | 1 | drop | protein | 2 (mM) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | reservoir | 0.2 (M) | ||
| 5 | 1 | reservoir | MPD | 35 (%) |
