1DUO
SPERM WHALE METAQUOMYOGLOBIN PROXIMAL HISTIDINE MUTANT H93G WITH 1-METHYLIMIDAZOLE AS PROXIMAL LIGAND.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1994-11-29 |
Detector | SDMS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.161, 49.033, 79.240 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.181 |
RMSD bond length | 0.020 |
RMSD bond angle | 3.000 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.150 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.182 |
Number of reflections | 9290 | |
<I/σ(I)> | 9.7 | |
Completeness [%] | 76.0 | 71.1 |
Redundancy | 2.19 | 1.36 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 35 % PEG 8000, 0.3 M NaOAc, 0.1 M PIPES, and 0.1 % dioxane, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Mb solution | 1 (%) | |
2 | 1 | reservoir | PEG8000 | 35-37.5 (%) | |
3 | 1 | reservoir | 0.3-0.35 (M) | ||
4 | 1 | reservoir | PIPES | 0.1 (M) | |
5 | 1 | reservoir | dioxane | 0.1 (%) |